期刊论文

Effects of electroacupuncture at Neiguan point on SOD and mitochondrion membrane potential in rabbits with myocardial ischemia-reperfusion injury

中文

湖南中医药大学学报

1674-070X

2015

35

7

47-49,53

10.3969/j.issn.1674-070X.2015.07.016

81072868:国家自然科学基金 81102661:国家自然科学基金 2013sk3086:湖南省科技厅项目

本校为第一机构

目的 研究电针内关穴预处理对心肌缺血再灌注损伤（MIRI）家兔线粒体通透性转换孔（m PTP）开放的影响,及其心肌保护作用机制。方法 新西兰大耳白兔18只随机分为假手术组、模型组、电针组,每组6只。采用冠脉结扎法造模,电针内关组在造模前,给予电针刺激20 min/d,共5 d10采用黄嘌呤氧化酶法测定血清超氧化物歧化酶（SOD）活性,荧光技术测定心肌细胞线粒体膜电位变化,分光光度法测定520 nm处线粒体吸光度的变化反映线粒体m PTP的开放,原位末端标记法测细胞凋亡。结果与模型组比较,电针组心肌细胞SOD活力显著提高,凋亡指数降低（P〈0.01）,线粒体膜电位显著提高（P〈0.05）,线粒体吸光度明显降低（...

Objective To investigate the cardioprotective mechanisms of electroacupuncture （EA） at Neiguan point on the opening of mPTP with in rabbits with myocardial isehemia-reperfusion injury （MIRI）. Methods 18 New Zealand rabbits were randomly divided into the sham group, model group and EA group, 6 rats in each group. The models were established by ligating the coronary artery. The EA group was given electroacupuncture stimulation pretreatment 20 rain every day lasting for 5 days before establishing MIRI model. The serum SOD activity was determin...