期刊论文

Effect of Fuke Qianjin Tablets on TLR2/4-NF-κB Signaling Pathway in Pelvic Inflammatory Model Rats

中文

湖南中医药大学学报

1674-070X

2016

36

11

13-17

10.3969/j.issn.1674-070X.2016.11.004

湖南省科技厅资助项目（2014SK3005）；

本校为第一机构

目的：观察妇科千金片对盆腔炎大鼠子宫 Toll 样受体2（ TLR2）、Toll 样受体4（ TLR4）、核转录因子 kappaB （ NF-κB ） mRNA 表达的影响，探讨妇科千金片调控 TLR2/4-NF-κB 信号通路对盆腔炎大鼠的抗炎机制。方法通过子宫注射混合菌液建立盆腔炎大鼠模型，将60只雌性 SD 大鼠随机分为5组，每组12只，即：空白组、假手术组、模型组、妇科千金片组、罗红霉素组，分别予以相应药物干预，运用免疫组化检测子宫组织中 TLR2、TLR4、NF-κB 的灰度值；运用实时荧光定量 PCR 检测子宫组织中 TLR2、TLR4、NF-κB 的 mRNA 表达变化。结果与空白组、假手术组比较，模型组的 TLR2、TLR4、NF-κB 灰度值...

Objective To observe the effect of Fuke Qianjin tablets on the expression of TLR2, TLR4, NF-κB in pelvic inflammatory model rats, and to investigate its anti-inflammatory mechanism of TLR2/4-NF-κB signaling pathway on the pelvic inflammatory rats. Methods The 60 female SD rats were injected with the mixture of the uterus to establish the model of the rats with pelvic inflammatory disease. Then the rats were randomly divided into 5 groups, 12 rats in each group, namely: blank group, sham operation group, model group, Fuke Qianjin tablets group...