期刊论文

中文

湖南中医药大学学报

1674-070X

2017

37

7

704-707

10.3969/j.issn.1674-070X.2017.07.003

81373577:国家自然科学基金 81403384:国家自然科学基金 15B151:湖南省教育厅优秀项目

本校为第一机构

目的 通过观察舒胃汤对功能性消化不良(functional dyspepsia,FD)肝郁脾虚证模型大鼠胃窦和幽门区Bcl-2、Caspase-12蛋白及m RNA表达的影响,探讨舒胃汤治疗FD的作用和机制。方法 将Wistar大鼠60只随机分成空白组,模型组,莫沙必利组,舒胃汤低、中、高剂量组。按改良复合病因造模法造成FD肝郁脾虚证模型,连续21 d。分组干预,连续14d。处死大鼠后,取胃窦平滑肌和十二指肠上段组织。Western-blot法检测Bcl-2、Caspase-12蛋白表达;RT-PCR法检测Bcl-2、Caspase-12的m RNA表达。结果 Western-blot检测发现,与空白组比,模型组Caspase-12表达升高而Bcl-2表达降低(P

Objective To observe the effect of Shuwei decoction on the expression of Bcl-2, Caspase-12 protein and mRNA in gastric antrum and pylorus of FD (functional dyspepsia) model rats with liver-stagnation and spleen-deficiency syndrome, and to explore the effect and mechanism of Shuwei decoction on FD. Methods The 60 Wistar rats were random-ly divided into blank group, model group, mosapride group, Shuwei decoction low, medium and high dose groups. FD syn-drome of liver stagnation and spleen deficiency was established by the method of improved complex etiology, 21 continu-ous days. Each group of ra...