期刊论文

中文

湖南中医药大学学报

1674-070X

2017

37

5

477-480

10.3969/j.issn.1674-070X.2017.05.004

14A107:湖南省教育厅重点项目 201429:湖南省中医药科研项目 2013135:湖南省中医药科研项目 15C1041:教育厅项目 2016T90752:中国博士后科学基金 2014M550420:中国博士后科学基金

本校为第一机构

目的 探讨乌药水提液对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠血清生长激素释放肽(Ghrelin)、胃动素(Motilin,MTL)、P物质(P substance,SP)、促胰液素(Secretin,Sec)水平的影响.方法 将60只大鼠随机分成6组,采用单只孤养1周+番泻叶煎剂灌胃2周+慢性束缚应激1周造模法建立IBS-D大鼠模型,分别给予乌药水提液低、中、高剂量,匹维溴铵灌胃干预.采用酶联免疫吸附法(ELISA)检测各组大鼠血清Ghrelin、MTL、SP、Sec含量.结果 与空白对照组比较,模型对照组SP、Ghrelin、MTL水平显著升高,Sec水平降低(P

Objective To investigate the water extracts of Lindera aggregata on Ghrelin, MTL, SP and Sec levels in rats with diarrhea-predominant irritable bowel syndrome. Methods 60 rats were randomly divided into 6 groups. Using a single solitary 1 weeks, gavage of sennoside for 2 weeks and chronic restraint 1 weeks to establish the rat model of IBS-D. The model rats were administrated with low, medium, high dose of water extracts of Lindera aggregata, and pinaverium bro-mide. The levels of SP, MTL, Sec and Ghrelin in serum of rats were measured by using enzyme linked immunosorbent assay (ELISA). Result...