Objective To explore the impact of long-term consumption of soybean on the intestinal microbial, and to pro-vide a basis for the daily diet. Methods The mice were divided into three groups of 1 month old (infancy), 6 months old (mid-dle aged) and 9 months old (old age) according to the age, and each group was randomly divided into control group, 1:1 group, 1:2 group and 1:4 group. The control group was fed a standard diet, 1:1,1:2 and 1:4 group were fed by 1:1,1:2 and 1:4 ratio of basic feed and soybean, respectively. After feedi...