期刊论文

Effect of Zhuanggu Zhitong Capsule Medicated Serum on the CDK4 and p21 Expression of Osteoblasts

中文

湖南中医药大学学报

1674-070X

2017

37

10

1078-1081

10.3969/j.issn.1674-070X.2017.010.007

14B130:湖南省教育厅科学研究优秀青年项目 2015217:湖南省大学生研究性学习和创新性实验计划 2015220:湖南省大学生研究性学习和创新性实验计划 201612:湖南省中医药科研重点项目

本校为第一机构

目的 观察壮骨止痛胶囊含药血清对成骨细胞CDK4和p21表达的影响,探讨壮骨止痛胶囊治疗骨质疏松症的机制.方法 40只SD雌性大鼠随机分为模型组、阳性对照组、壮骨止痛胶囊组、假手术组,每组10只.模型组、阳性对照组以及壮骨止痛胶囊组的SD大鼠制作骨质疏松大鼠模型,假手术组SD大鼠作为对照.不同组SD大鼠喂养不同药物,假手术组和模型组大鼠灌胃生理盐水,阳性对照组灌胃尼尔雌醇,壮骨止痛胶囊组灌胃壮骨止痛胶囊药物,连续喂养13周之后,处死大鼠,提取各组大鼠含药血清备用.另取5只新生SD小鼠颅骨的成骨细胞,应用上述各组含药血清进行细胞培养,连续培养3代后,采用碱性磷酸酶染色和茜素红染色法鉴定成骨...

Objective To obseve the effect of Zhuanggu Zhitong capsule medicated serum on the CDK4 and p21 ex-pression of osteoblasts, and investigate its mechanism of action on osteoporosis. Methods Forty female SD rats were random-ly divided into model group, positive control group, Zhuanggu Zhitong capsule group and sham-operation group, 10 rats in each group. The SD osteoblasts model rats excluding rats in sham-operation group were built, and shamp-operation group as the control. Rats in sham-operation and model groups were given normal saline by gavage, rats in the positive control group were adminis...