期刊论文

Effects of Electroacupuncture at Neiguan Point on Mitochondrion Membrane Potential and Adenosine A1 Receptor in Rats with Myocardial Ischemia-Reperfusion Injury

中文

湖南中医药大学学报

1674-070X

2017

37

4

402-405

10.3969/j.issn.1674-070X.2017.04.016

81574080:国家自然科学基金 2015211:湖南省中医药局项目 15k093:湖南省高等学校创新平台开放基金 :针灸推拿学湖南省"十二五"重点学科开放基金资助项目

本校为第一机构

目的 观察电针内关预处理对心肌缺血再灌注损伤（myocardial ischemic reperfusion injury,MIRI）大鼠心肌线粒体膜电位、腺苷A1受体的影响,探讨电针预处理防治MIRI的可能作用机制.方法 40只SD雄性大鼠随机分为假手术、缺血再灌注组（模型组）、电针内关穴组、电针环跳穴组,每组10只.采用冠脉结扎法造模,电针内关组和电针环跳穴组在造模前,给予电针刺激20 min/d,共7 d.采用荧光技术测定心肌细胞线粒体膜电位变化,免疫组化法测腺苷A1受体的表达.结果模型组线粒体膜电位及腺苷A1受体较假手术组明显下降（P〈0.01）;电针内关穴组线粒体膜电位较模型组、电针环跳穴组及假手术组明显升高（P〈0.05）;...

Objective To explore the cardioprotective mechanisms of electroacupuncture (EA) at Neiguan point and ob-serve the effect of mitochondrial membrane potential and adenosine A1 receptors in rats with myocardial ischemia-reperfusion injury. Methods The 40 SD male rats were randomly divided into the sham-operation group, ischemia reperfusion (mod-el group), EA Neiguan group and EA Huantiao group, 10 rats in each group. The models were established by ligating the coronary artery. The EA Neiguan group and EA Huantiao group were given electroacupuncture stimulation pretreatment 20 min every day lastin...