期刊论文

Isolation,Culture and Identification of Interstitial Cells of Cajal in Gastric Antrum of Adult Rats

中文

湖南中医药大学学报

1674-070X

2017

37

11

1226-1230

10.3969/j.issn.1674-070X.2017.11.014

国家自然科学基金项目（81403487） 湖南省教育厅青年基金（14B128）

本校为第一机构

目的 探讨成年SD大鼠胃窦起搏细胞Cajal间质细胞(interstitial cells of cajal,ICC)的原代分离、培养及鉴定方法,为深入研究其生理功能提供条件.方法 8~9周龄SD成年大鼠,禁食24 h,乙醚吸入麻醉,颈椎脱臼处死.无菌条件下采用精细解剖方法快速取出胃窦平滑肌组织,将其剪成1~2 mm3小块后,使用II型胶原酶消化法于37℃培养箱内消化60 min,离心后过200目筛,接种于DMEM/F12完全培养基(含5 ng/mL干细胞因子)中进行培养.用酪氨酸蛋白激酶受体c-kit特异性抗体免疫荧光染色鉴定细胞类型.结果 培养72 h后,于倒置显微镜下观察,可见细胞贴壁,呈不规则三角形、星形或梭形,有多个突起;随着培养时间的延长,各突起...

Objective In order to provide the conditions for further study of its physiological function, the method of isolation, culture and identification of interstitial cells of Cajal (ICC) in the gastric antrum of adult SD rats was investigated. Methods SD rats at 8-9 weeks, were anesthetized with ether and killed by cervical dislocation. The specimens were cut into 1-2mm3 small pieces, digested with collagenase type II in a 37 ℃ incubator for 60 min, centrifuged and passed through a 200-mesh sieve. The tissues were inoculated in DMEM / F12 complete...