期刊论文

Protective Effect and Mechanism Research of Panax Notoginseng Saponins Pretreatment on Renal Ischemia Reperfusion Injury of Rats

中文

湖南中医药大学学报

1674-070X

2018

38

1

21-26

10.3969/j.issn.1674-070X.2018.01.006

81503442:国家自然科学基金 81603470:国家自然科学基金 ZXJCXK201503:湖南省重点学科建设项目(十二五) 2015-216:湖南省大学生研究性学习和创新性实验计划 17B197:湖南省教育厅优秀青年项目 16B196:湖南省教育厅优秀青年项目

本校为第一机构

目的 探讨三七总皂苷对大鼠肾缺血再灌注损伤(renal ischemia reperfusion injury,RIRI)的保护作用,并研究其对氧化应激及炎症级联反应的影响.方法 将40只雄性SD大鼠随机均分为假手术组,模型组,三七总皂苷低、中、高剂量[40、80、160 mg/(kg·d)]预处理组,分别予以相应干预,连用5 d.在最后1次给药1 h后采用切除右肾、夹闭左肾动脉45 min后再灌注3 h的方法建立RIRI模型.五组均于再灌注3 h后经腹主动脉采血5 mL,检测血清中尿素氮(BUN)、 血肌酐(SCr)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;取肾组织常规切片和HE染色,光镜下观察肾组织病理学改变;免疫组化法检测细胞间黏附因子-1(ICAM-1)、核...

Objective To investigate the protective effect and its mechanism of panax notoginseng saponins (PNS) on re-nal ischemia reperfusion injury (RIRI) in rats. Methods 40 male SD rats were randomly divided into the sham-opera-tion group, model group, low dose, medium dose, high dose [40, 80, 160 mg/(kg·d)] PNS groups. All rats were administered for 5 d, respectively. After ending treatment for 1 h, the RIRI model was established. After reperfusion for 3 h, the levels of urine nitrogen (BUN), secrumcreatinine (SCr), the content of malondialdehyde (M...