期刊论文

An Improved Method for the Preparation of Para-chlorobenzalanine Suspension in a Rat Model of Insomnia

中文

湖南中医药大学学报

1674-070X

2018

38

9

986-989

10.3969/j.issn.1674-070X.2018.09.004

国家重点基础研究发展计划（973计划）（2014CB543102）；

本校为第一机构

目的 探讨大鼠失眠模型造模中对氯苯丙氨酸(para-chlorophenylalanine,PCPA)混悬液配制的改良方法,并对造模效果进行评价. 方法 98 只 SD 大鼠中 50 只用 NaOH 溶液配制法、24 只用 NaHCO3 溶液配制法和 24 只用改良法进行失眠大鼠造模,探讨制作 PCPA 致失眠模型的最佳方法. 结果 NaOH 溶液配制法 50 只大鼠造模失败,NaHCO3 溶液配制法 24 只大鼠造模失败,改良法 24 只大鼠造模成功,且 PCPA 剂量以 0.5 g/kg 造模效果最好(P＜0.05). 结论 改良的 PCPA 混悬液的配制法可以成功制作大鼠失眠模型.

Objective To explore the improved method of preparing para -chlorophenylalanine (PCPA) suspension in a rat model of insomnia, and evaluate the effect of modeling. Methods In the 98 SD rats, 50 rats were treated with NaOH solution preparing method, 24 SD rats were modeled with NaHCO3 solution preparing method, and 24 SD rats were treated with the improved method to establish the model of insomnia rats. Results There were 50 failed model rats with NaOH solution preparing method, 24 failed model rats with NaHCO3 solution preparing method. The 24 m...