期刊论文

中文

湖南中医药大学学报

1674-070X

2018

38

1

36-39

10.3969/j.issn.1674-070X.2018.01.009

湖南省教育厅研究生创新课题（CX2015B338）； 湖南省中医药管理局一般项目（201653）；

本校为第一机构

目的 探讨葛根素对大鼠脑缺血再灌注损伤后海马组织磷酸化的转录激活蛋白3(STAT3)、凋亡因子P53表达的影响及其保护机制.方法 采用线栓法制备大鼠左侧大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,利用葛根素干预,分别于缺血2 h再灌注6 h、24 h、72 h 3个不同时间点采用Longa评分法进行神经功能缺损评分、TUNEL法检测凋亡细胞数,免疫组织化学法检测海马组织磷酸化的STAT3(P-STAT3)及细胞凋亡因子P53的表达.结果 与假手术组比较,葛根素组与缺血再灌注组的神经功能缺损加重,凋亡细胞数增多,P-STAT3和P53表达增高,差异均具有统计学意义(P

Objective To observe the effect of puerarin on the expression of phosphorylated transcription activator protein three(STAT3)and apoptosis factor P53 in the hippocampus tissue of cerebral ischemia reperfusion injury rats and to investigate its mechanism.Methods The middle cerebral artery occlusion(MCAO)model of left cerebra in rats were prepared by using suture method.The rats were given puerarin to intervene at 6 h,24 h,72 h for reperfusion after ischemia for 2 h.The neurologic impairment score was evaluated by Longa scoring,apoptotic cells number was detected by TUNEL method,and the expressio...