期刊论文

Effect of Acupuncturing Dazhui,Baihui and Renzhong Acupoints on Expression of Neuroglobin at Different Time Points in Hippocampus of Rats with Cerebral Ischemia Reperfusion Injury

中文

湖南中医药大学学报

1674-070X

2018

38

2

165-168

10.3969/j.issn.1674-070X.2018.02.011

81303051:国家自然科学基金 2016JJ3101:湖南省自然科学基金 ZYNK201501:省部共建教育部重点实验室基金

本校为第一机构

目的 观察针刺大椎、百会、人中穴对大鼠脑缺血再灌注损伤后不同时间点海马脑红蛋白(neuroglobin,Ngb)表达的影响,探讨其脑保护作用的部分作用机制.方法 采用线栓法制备MCAO模型,将40只雄性SD大鼠随机分为假手术组、模型组、对照点组、穴位组4大组,每组再根据再灌注后时间分为24 h、72 h组,每组5只.完成治疗后,先行神经功能缺损评分再处死大鼠,再采用Western Blot法检测大鼠缺血侧海马Ngb的表达水平.结果 神经功能缺损评分:与假手术组比较,模型组、对照点组及穴位组神经功能缺损评分均升高,差异有统计学意义(P0.05);72 h对照点组及穴位组神经功能缺损评分均下降,差异有统计学意义(P

Objective To observe the effects of acupuncturing Dazhui, Baihui and Renzhong acupoints on hippocampus neuroglobin (Ngb) expression at different time points in rats with middle cerebral artery occlusion (MCAO), and to explore the part mechanism of brain protection. Methods MCAO model was established by thread embolism method. 40 male SD rats were randomly divided into 4 groups: sham operation group, model group, control point group and acupoint group. Each group was then divided into two groups according to reperfusion time at 24 h and 72 h, 5 rats in each group. After treatment, the nerve fun...