期刊论文

In vitro Antibacterial Activity of the Part of Wuwei Xiaodu Decoction Separated by Macroporous Resin

中文

湖南中医药大学学报

1674-070X

2018

38

8

858-861

10.3969/j.issn.1674-070X.2018.08.004

国家自然科学基金项目（81703985）； 湖南省教育厅科研项目（14C0860）；

本校为第一机构

目的 检测五味消毒饮不同分离部位的体外抗菌活性,为五味消毒饮的二次开发提供实验依据.方法 本研究将五味消毒饮水煎液用NKA-9大孔树脂进行吸附分离得到不同部位的洗脱液,用牛津杯法测定其抑菌圈,并用试管稀释法检测洗脱液对细菌的最低抑菌浓度(MIC).结果 各分离部位对不同菌株的抑菌效果有显著差异,30％乙醇洗脱液对金黄色葡萄球菌和志贺氏痢疾杆菌的抑制效果最好,MIC分别为0.055 g/mL和0.150 g/mL;而水洗液对肺炎链球菌杆菌的抑制效果最佳,MIC为0.184 g/mL,对金黄色葡萄球菌和志贺氏痢疾杆菌没有抑制作用;水洗液、10％乙醇洗脱液、30％乙醇洗脱液对绿脓杆菌几乎没有抑制效果,极性稍小的50％乙...

Objective To investigate the in vitro antibacterial activity of different separated parts of Wuwei Xiaodu Decoction, and to provide an experimental basis for the secondary development of Wuwei Xiaodu Decoction. Methods The water extract solution of Wuwei Xiaodu Decoction was absorbed and separated by NKA-9 macroporous resin to obtain the eluent of each part. The Oxford cup method was used to measure the inhibition zone, and the tube dilution method was used to measure the minimal inhibitory concentration （MIC） of the eluent. Results There was...