期刊论文

中文

湖南中医药大学学报

1674-070X

2018

38

3

257-260

10.3969/j.issn.1674-070X.2018.03.007

国家自然科学基金（81302953）； 湖南省中医药科研计划项目（2014136）； 湖南省教育厅科研项目（15C1050）； 中药粉体与创新药物省部共建国家重点实验室培育基地开放基金（ZYFT201510）；

本校为第一机构

目的 探讨补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因Bcl-2、Bax的影响.方法 将SD大鼠80只,雌雄各半,复制局灶性脑缺血大鼠模型,造模后按神经功能缺损评分进行分层,随机分为5组,每组15只,分别于移植后7d、14 d、28 d进行神经功能评分,采用补阳还五汤超微饮片与传统饮片予以干预,于相应时间点处死大鼠后采用免疫组化法检测凋亡基因Bcl-2、Bax.结果 补阳还五汤能显著减轻神经功能缺损,Bcl-2、Bax在各时间点(7 d、14 d、28 d)表达与模型组比较差异有统计学意义(P

Objective To investigate the effects of Buyang Huanwu ultra-fine powder on apoptosis protein of Bcl-2 and Bax in transplanted neural stem cells in rats with focal cerebral ischemia. Methods Eighty Sprague-Dawley rats, half male and half female, were copyed the focal cerebral ischemia models, which were randomly divided into five groups, 15 rats in each group. The model rats were intervened by using Buyang Huanwu ultra-fine powder and traditional decoction. At 7 d,14 d and 28 d of post-tansport, the neural function defect scale was measured. The rats were sacrified at according time. Bcl-2 and ...