期刊论文

Proteomic Analysis of Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats

中文

湖南中医药大学学报

1674-070X

2018

38

6

605-613

10.3969/j.issn.1674-070X.2018.06.001

国家自然科学基金项目（81774207;81202647;81473616）； 中医诊断国家重点学科开放项目（2014-16）；

本校为第一机构

目的 研究自发性高血压大鼠(spontaneously hypertensive rats,SHR)左心室肥厚心肌组织的蛋白质变化.方法 对8只SHR与8只WKY大鼠组织蛋白质进行iTRAQ检测分析;通过对差异蛋白Pathway富集分析选择与通路相关的蛋白质.结果 筛选出与通路相关的40个差异蛋白,SHR与WKY比较上调的差异蛋白有:1主要组织相容性复合物、2血小板活化因子乙酰水解酶、3有丝分裂原蛋白激酶、4热休克27kDa蛋白、5 Ighg3、6 14-3-3γ/蛋白、7纤维蛋白原、8蛋白酶体26S、9纤维蛋白原γ链、10中性胆固醇酯水解酶、11溶血PAF乙酰转移酶、12纤连蛋白.下调的差异蛋白有:13丝氨酸蛋白酶、14载脂蛋白H、15血清锌α2糖蛋白、16泛醌9、...

Objective To study the protein changes in the myocardium of left ventricular hypertrophy inspontaneously hypertensive rats （SHR）. Methods The protein of 8 SHR and 8 WKY rats were detected and analyzed byiTRAQ detection and analysis technique. The proteins related to pathway were selected by enrichment analysis of differentialprotein pathway. Results The 40 differential proteins related to pathways were screened. Upregulated proteins in SHR comparedwith WKY： 1 major histocompatibility complex, 2 platelet activating factor acetylhydrolase, 3 m...