期刊论文

Study of the Activities of Antioxidant and Inhibiting Differentiation of 3T3-L1 Preadipocytes of the Extract from Phragmites australis(Cav.)Trin.ex Steud

中文

湖南中医药大学学报

1674-070X

2018

38

1

27-30

10.3969/j.issn.1674-070X.2018.01.007

16K064:湖南省教育厅科学研究项目 kq1602015:长沙市科技局项目 :湖南省芦笋研究开发中心芦笋产业联合研究开发基金

本校为第一机构

目的 研究芦笋提取物的抗氧化能力和抑制3T3-L1前脂肪细胞分化的活性.方法 采用乙醇回流提取,通过酸碱处理,制备得到芦笋提取物;利用紫外分光光度法测定提取物中的酚酸含量(以没食子酸计);采用DPPH法测定提取物清除自由基的活性;通过CCK8法及油红O染色法分别检测芦笋提取物对3T3-L1前脂肪细胞存活率及分化率的影响.结果芦笋提取物中以没食子酸计的酚酸含量为0.5308 mg/mg;测得提取物清除自由基活性强弱与剂量成正相关,当其浓度达到2 mg/mL时,其对DPPH的清除率可达89.68％;在10～200μg/mL的测定浓度范围内,提取物对3T3-L1细胞生存率无明显影响,对3T3-L1细胞的分化有明显抑制作用,且呈剂量依赖性...

Objective To study the activities of antioxidant and inhibiting differentiation of 3T3-L1 preadipocytes of Lusun [Phragmites australis (Cav.) Trin. ex Steud] extract. Methods The Lusun exatract was prepared by using ethanol re-flux extraction and acid and alkali treatment. The content of phenolic acid of the extract was mearsured by UV spectropho-tometry. The activity of free radical scavenging of extract was measured by using DPPH method. The effects of the extract on the survival rate and differentiation rate of 3T3-L1 preadipocytes were dete...