期刊论文

Effect of Heshouwu Granule on Learning and Memory and miRNA-101 Expression in a Rat Model of Alzheimer＇s Disease

中文

湖南中医药大学学报

1674-070X

2018

38

10

1120-1124

10.3969/j.issn.1674-070X.2018.10.006

湖南省中医药管理局资助课题（2015107）；

本校为第一机构

目的 研究何首乌对阿尔茨海默病模型大鼠学习记忆能力及双侧海马区miRNA-101表达的影响,并探讨其作用机制.方法 将120只SD大鼠随机分为4组,每组30只,分别为假手术组、石杉碱甲组、模型组、中药组.除假手术组外,使用化学试剂冈田酸(OA)注射SD大鼠杏仁核部位,形成阿尔茨海默病模型.造模后每组分别予以相应干预(假手术组与模型组均灌等容量生理盐水).干预21 d结束后,运用Morris水迷宫作为行为学检测方法,评测大鼠学习记忆能力,行为学观察共进行5 d,结束后处死实验大鼠,取双侧海马运用RT-PCR进行miRNA-101半定量检测.结果 经21 d干预后,与模型组相比,中药组与石杉碱甲组的阿尔茨海默病模型大鼠的学习...

Objective To study the effect of Fallopia multiflora on learning and memory abilities and miRNA-101 expression in bilateral hippocampus in a rat model of Alzheimer＇s disease, and to explore its mechanism. Methods A total of 120 Sprague-Dawley rats were randomly and equally divided into four groups： sham-operation group, control （huperzine A） group, model group, and traditional Chinese medicine （TCM） group. To establish a model of Alzheimer＇s disease, okadaic acid was injected into the amygdala of rats in all the groups except for the sha...