期刊论文

中文

湖南中医药大学学报

1674-070X

2018

38

11

1273-1277

10.3969/j.issn.1674-070X.2018.11.011

16K068:湖南省教育厅创新平台项目 017-132:湖南省大学生创新实验项目

本校为第一机构

目的 观察隔药饼灸对慢性支气管炎大鼠肺组织中肿瘤坏死因子-α (TNF-α) 蛋白及其mRNA含量的影响.方法 将48只SD大鼠随机分为空白组、模型组、隔药饼灸组、隔纸垫灸组, 每组12只.采取改良烟熏法复制慢性支气管炎大鼠模型, 观察用隔药饼灸治疗后大鼠的一般情况, 并与空白组、模型组、隔纸垫灸组进行对照, 大鼠肺组织中的TNF-αmRNA的表达用RT-PCR法检测, 肺组织中TNF-α的蛋白表达用Western blot法检测.结果 与模型组相比, 隔药饼组和隔纸垫组大鼠肺及气管的病理损伤均有好转;隔药饼灸组和隔纸垫灸组都能使慢支大鼠肺组织TNF-αmRNA及TNF-α蛋白含量表达降低 (P＜0.05), 且隔药饼灸组效果更好 (...

Objective To observe the effect of herbal cake-separated moxibustion on the content of tumor necrosis factor-α (TNF-α) protein and its mRNA in the lung tissue of rats with chronic bronchitis (CB). Methods Forty-eight Sprague-Dawley rats were randomly and equally divided into four groups, including blank group, model group, herbal cake-separated moxibustion group, and paper pad-separated moxibustion group. CB model rats were prepared by modified fumigation. The general status of rats after herbal cake-separated moxibustion was observed and com...