期刊论文

Effect of Ziyin Mingmu Pill on the Expression of Bax and Caspase-3 in the Retina in Royal College Surgeons Rats

中文

湖南中医药大学学报

1674-070X

2018

38

7

732-737

10.3969/j.issn.1674-070X.2018.07.004

2015ZYZD02:湖南中医药大学中医诊断学国家重点学科开放基金 16K065:湖南省教育厅创新平台开放基金 :湖南省***卫生人才225工程培养项目 :国家中医药管理局中医药重点学科建设 :湖南省中医五官科学重点学科建设项目

本校为第一机构

目的观察滋阴明目丸对皇家外科学院（royal college surgeons,RCS）大鼠视网膜Bax及Caspase-3表达的影响。方法实验对象分3组,分别为:空白组、模型组、滋阴明目丸组（每组雌雄各4只共8只）。空白组:RCS（rdy+/+,p+/+）大鼠,灌胃生理盐水;模型组:RCS（rdy-/-,p-/-）大鼠,灌胃生理盐水;滋阴明目丸组:RCS（rdy-/-,p-/-）大鼠,灌胃滋阴明目丸混悬液。灌胃30 d后,免疫组化法检测各组大鼠视网膜组织中Bax及Caspase-3表达,RT-q PCR法及Western Blot法分别测定各组视网膜组织中Bax及Caspase-3 m RNA及蛋白相对表达水平。结果 Bax、Caspase-3蛋白在滋阴明目丸组视网膜中的阳性表达显著低于模型组,但高于空...

Objective To investigate the effect of Ziyin Mingmu Pill on the expression of Bax and Caspase-3 in the retina in Royal College Surgeons(RCS)rats.Methods RCS rats were divided into blank group,model group,and Ziyin Mingmu Pill group,with 8 rats(4 male and 4 female rats)in each group.The RCS(rdy+/+,p+/+)rats in the blank group were treated with normal saline by gavage;the RCS(rdy-/,p-/-)rats in the model group were given normal saline by gavage;the RCS(rdy-/,p-/-)rats in the Ziyin Mingmu Pill group were given the suspension of Ziyin Mingmu Pill by gavage.After 30 days of intragastric administrat...