期刊论文

A Method for Establishing Young Rat Model of Asthma Induced by Respiratory Syncytial Virus Combined with Ovalbumin

中文

湖南中医药大学学报

1674-070X

2018

38

12

1371-1373

10.3969/j.issn.1674-070X.2018.12.004

河北省重点研发计划项目（17277713D）； 湖南省自然科学基金项目（2015JJ2016）；

本校为第一机构

目的探讨呼吸道合胞病毒（respiratory syncytial virus, RSV）滴鼻联合卵清白蛋白（ovalbumin, OVA）雾化方法,建立大鼠哮喘模型的可行性。方法将SD大鼠共30只随机分为正常对照组（A组）、哮喘模型组（B组）和地塞米松组（C组）3组,每组10只,分别给予相应干预,两周后观察大鼠反应,应用肺功能测量仪测定气道阻力（Re值）、支气管肺泡灌洗液（bronchoalveolar lavage fluid, BALF）中细胞计数与分类,并观察肺组织病理改变。结果 A组大鼠反应及Re值基本正常,B组大鼠出现喘息表现,且测Re值明显高于A组（P

Objective To investigate the feasibility of intranasal delivery of respiratory syncytial virus(RSV)with ovalbumin(OVA)atomization to establish a rat model of asthma.Methods A total of30Sprague-Dawley rats were equally and randomly divided into group A(normal control group),group B(asthma model group),and group C(dexamethasone group)to receive respective treatments.After two weeks,the reaction of rats was observed.Airway resistance(Re value)was determined using a pulmonary function meter.Cell counting and classification were performed for bronchoalveolar lavage fluid(BALF).The pathological chan...