Objective To study the chemical constituents of Achyranthes aspera L. produced in Hunan. Methods The constituents were separated and purified by silica gel and ODS column chromatography and their structures were identified on the basis of physicochemical properties, spectral data and reference. Results Seven compounds were isolated and identified as β-ecdysterone(1), inokosterone (2), polypodine B (3), pterosterone (4), 24(28)-ecdysterone (5), achyranthesterone A (6), rubrosterone (7). Conclusion Compounds 4-7 were ...