期刊论文

Effects of Bushen Huoxue Decoction onβ-catenin Protein Level of WNT Signal Pathway in Lumbar Intervertebral Disc Degeneration Aging Model Rats

中文

湖南中医药大学学报

1674-070X

2018

38

3

275-279

10.3969/j.issn.1674-070X.2018.03.011

湖南省中医药管理局资助项目（201221）；

本校为第一机构

目的 观察补肾活血汤对衰老大鼠退变椎间盘组织形态结构及β-catenin蛋白含量的影响.方法 选取三月龄健康SD大鼠80只,雌雄各半,随机分为空白组、假模型组、模型组、中药低剂量组以及中药高剂量组,每组16只,雌雄各半.除空白组和假模型组外,其余各组注射D-半乳糖建立大鼠衰老动物模型,空白对照组、假模型组与模型组每日生理盐水灌胃,中药低剂量组及中药高剂量组补肾活血汤每日灌胃,持续12周.分别于造模后、灌胃4周后、灌胃8周后、灌胃12周后每组随机抽取雌雄大鼠各2只处死取材,光镜下观察椎间盘组织形态学变化并运用蛋白质印迹法(Western Blot)检测β-catenin蛋白表达差异.结果 与模型组比较,经补...

Objective To observe the effect of Bushen Huoxue decoction on the structural and histopathological changes and β-catenin protein in lumbar intervertebral disc degeneration aging model rats. Methods Eighty three-month old SD rats(male-female half and half) were randomly divided into blank control group, sham-model group, model group, TCM low-dose group and TCM high-dose group, 16 rats in each group. Except the blank group and sham-model group, the aging model rats in other groups were established by injecting D-galactose. The rats in blank cont...