期刊论文

中文

湖南中医药大学学报

1674-070X

2019

39

10

1195-1199

10.3969/j.issn.1674-070X.2019.10.005

湖南省自然科学基金项目(2016JJ6082) 湖南省中医药管理局重点项目(201634).

本校为第一机构

目的 研究中药疏肝健脾解毒方对乳腺癌癌前病变肝郁证大鼠模型乳腺组织微血管密度(microvessel density,MVD)、血管内皮生长因子(vascular endothelial growth factor,VEGF)及乳腺组织结构的影响,探讨其防治乳腺癌的作用机制.方法采用二甲基苯蒽灌胃及夹尾法制备乳腺癌癌前病变肝郁证大鼠模型,随机分为模型组(蒸馏水)、中药组(疏肝健脾解毒方10.35 g/kg)、三苯氧胺组(2.0 mg/kg),另取10只为空白对照组,分别相应干预4周后,制备乳腺组织石蜡切片进行病理学观察,采用免疫组织化学方法及Western-blot法检测各组大鼠乳腺组织中MVD及VEGF表达情况.结果免疫组化结果显示,与模型组比较,中药组及三苯氧胺...

Objective To study on effects of Shugan Jianpi Jiedu Formula on microvessel density (MVD), vascular endothelial growth factor (VEGF) and breast tissue structure in breast cancer rat model with precancerous lesion of liver Qi stagnation syndrome, so as to explore its mechanism in prevention and treatment of breast cancer. Methods The breast cancer rat model with precancerous lesion of liver Qi stagnation syndrome was prepared by intragastric administration of dimethyl benzanthracene and tail clamping method. The rats were randomly divided into a model group (distilled water), a Chinese materia ...