期刊论文

Protective Effect of Thin Recipe of Buyang Huanwu Decoction Against Oxidative Stress Injury in Vascular Endothelial Cells

中文

湖南中医药大学学报

1674-070X

2019

39

2

163-167

10.3969/j.issn.1674-070X.2019.02.004

81603415:国家自然科学基金 2017JJ3231:湖南省自然科学基金 201820:湖南省中医药局重点项目 15C1034:湖南省教育厅一般项目 17C1648:湖南省教育厅一般项目

本校为第一机构

目的 观察补阳还五汤精简方对氧化应激损伤后血管内皮细胞的保护作用,并从核转录因子E2相关因子/抗氧化反应元件(NF-E2-related factor 2/antioxidant response element,Nrf2/ARE)途径探讨其作用机制.方法 采用H2O2作用大鼠血管内皮细胞4 h建立体外血管内皮细胞氧化应激模型,根据不同处理分成正常组,模型组,补阳还五汤含药血清组(补阳组),补阳还五汤精简方含药血清组(5％精简组、10％精简组、20％精简组)6组.采用倒置显微镜观察细胞形态;流式细胞术检测血管内皮细胞调亡;测定细胞SOD活力、MDA含量;采用Western blot法检测细胞Nrf2、血红素加氧酶-1(HO-1)蛋白的表达.结果 与模型组比较,含药血清组...

Objective To investigate the protective effect of thin recipe of Buyang Huanwu Decoction on vascular endothelial cells after oxidative stress injury and its mechanism of action based on the nuclear factor-erythroid 2-related factor 2(Nrf2)/antioxidant response element(ARE)pathway.Methods Rat vascular endothelial cells were treated with H2O2 for 4 hours to establish an in vitro model of oxidative stress in vascular endothelial cells,and according to the treatment method,the cells were divided into normal group,model group,serum containing Buyang Huanwu Decoction group(Buyang group),and serum co...