期刊论文

中文

湖南中医药大学学报

1674-070X

2019

39

3

320-325

10.3969/j.issn.1674-070X.2019.03.008

国家自然科学基金资助项目（81473564）；

本校为第一机构

目的 观察金水六君煎及其拆方含药血清对肺腺癌细胞A549黏液高分泌模型黏蛋白5AC(MUC5AC)及水通道蛋白5 (AQP5)表达的影响,探讨金水六君煎治疗气道黏液高分泌的作用机制.方法 将A549细胞分为空白组、模型组、金水六君煎组、养阴组、化痰组.采用人中性粒弹性蛋白酶(HNE)25 nmmol/L诱导A549细胞黏液高分泌,20%金水六君煎及其拆方含药血清干预24 h.RT-PCR、Western blot法检测细胞MUC5AC、AQP5基因与蛋白表达.ELISA法检测细胞上清液MUC5AC、AQP5蛋白表达.结果 与空白组比较,模型组细胞MUC5AC mRNA与蛋白表达明显增多(P＜0.05),AQP5 mRNA与蛋白表达明显降低(P＜0.05).与模型组比较,金水六君煎组及养...

Objective To investigate the effects of Jinshui Liujun Decoction and its components on the expression of mucin 5AC (MUC5AC) and aquaporin 5 (AQP5) in the mucus hypersecretion model of human lung adenocarcinoma A549 cells, and to explore the mechanism of action of Jinshui Liujun Decoction in the treatment of airway mucus hypersecretion. Methods A549 cells were divided into blank group, model group, Jinshui Liujun Decoction group, Yin-nourishing group, and phlegm-resolving group. Mucus hypersecretion in A549 cells was induced using human neutral elastase (HNE) at a concentration of 25 nmmol/L, f...