期刊论文

Metabolic Transformation of Berberine and Jatrorrhizine in Rat Liver Microsomes

中文

湖南中医药大学学报

1674-070X

2019

39

3

326-330

10.3969/j.issn.1674-070X.2019.03.009

天然药物活性物质与功能国家重点实验室开放课题（GTZK201607）； 湖南省教育厅优秀青年基金（2015B172）； 湖南省中医药科研基金（201494）； 湖南省“十二五”省级药学重点学科（1007）开放基金；

本校为第一机构

目的 进行小檗碱和药根碱在大鼠肝微粒体中的代谢转化研究,尝试给中药有效成分的体外和体内关联研究提供一定的参考.方法 制备大鼠肝微粒体,用Brad ford法测定酶活性,以此配制大鼠肝微粒体体外温孵体系,加入药物温孵,用质谱进行检测测定并比较小檗碱、药根碱代谢产物图谱.结果 检测到小檗碱经大鼠肝微粒体代谢后产物中有药根碱,药根碱经大鼠肝微粒体代谢后产物中有巴马汀,但小檗碱代谢产物中未见巴马汀.结论 小檗碱和药根碱是同母核生物碱,在肝微粒体中孵化可相互影响,但体外代谢无法简单关联体内代谢过程.

Objective To investigate the metabolic transformation of berberine and jatrorrhizine in rat liver microsomes, and to provide a reference for the in vitro and in vivo association studies of active constituents in traditional Chinese medicine (TCM).Methods The rat liver microsome was prepared, and the enzymatic activity was determined by Brad ford method to prepare the in vitro incubation system of rat liver microsomes.After the incubation in the presence of berberine and jatrorrhizine, mass spectrometry was used to detect and compare the metabolites of berberine and jatrorrhizine.Results After ...