期刊论文

Effects of Zuogui Jiangtang Jieyu Recipe on mEPSC of Hippocampal NVU Neurons in DD Rats

中文

湖南中医药大学学报

1674-070X

2020

40

7

823-827

10.3969/j.issn.1674-070X.2020.07.009

国家“重大新药创制”科技重大专项资助项目(2017ZX09309026) 国家自然科学基金项目(81874464,81703694) 中医学一流学科开放基金(2018ZYX50) 湖南省研究生创新项目(CX20190566) 湖南省湖南中医药大学研究生创新项目(2018CX70) 湖南中医药大学中医学国内一流学科资助项目 湖南中医药大学研究生创新课题(2018CX68)。

本校为第一机构

目的 观察左归降糖解郁方对糖尿病并发抑郁症(diabetes mellitus with depression,DD)大鼠海马神经血管单元(neu-rovascular unit,NVU)体外共培养体系的神经元微小兴奋性突触后电流(miniature excitatory postsynaptic currents,mEPSC)频率及幅度的影响,探讨其对海马NVU培养体系的保护机制.方法 有效构建海马NVU体外培养体系,采用免疫细胞化学染色法进行细胞鉴定;采用高糖(150 mmol/L)联合皮质酮(200μmol/L)干预构建海马NVU细胞模型;采用全细胞膜片钳记录NVU体外共培养体系神经元mEPSC,比较各组NVU培养体系神经元mEPSC频率和电流幅度.结果 经免疫细胞化学鉴定,NVU培养体系中细胞结构完整.与正常...

Objective To observe the effects of Zuogui Jiangtang Jieyu Recipe on the frequency and amplitude of miniature excitatory postsynaptic currents(mEPSC) in the hippocampal neurovascular unit(NVU) co-culture system in rats with diabetes mellitus with depression(DD), and to explore its protection mechanism for hippocampal NVU culture system.Methods The hippocampal NVU in vitro co-culture system was constructed efficiently.Cell identification was performed by immunocytochemical staining.Hippocampus NVU cell model was constructed by high glucose(150 m...