期刊论文

Effects of Zuogui Jiangtang Jieyu Fang on NR and mEPSC in Hippocampal Neurons of Fetal Rat with Diabetes Mellitus Complicated with Depression

中文

湖南中医药大学学报

1674-070X

2020

40

3

274-279

10.3969/j.issn.1674-070X.2020.03.006

81874464:国家自然科学基金 17C1210:教育厅项目 2018JJ3388:国家自然科学基金 :湖南中医药大学中医内科学重大疾病防治 ZYNK201608:教育部重点实验室开放基金 ZYNK201708:教育部重点实验室开放基金 2018ZYX49:湖南中医药大学中医学国内一流建设学科开放基金资助项目

本校为第一机构

目的 探讨左归降糖解郁方对模拟糖尿病并发抑郁(diabetes mellitus with depression,DD)症状态下胎鼠海马神经元N-甲基-D-天冬氨酸受体(n-methyl-d-aspartic acid receptor,NR)和微小兴奋性突触后电流(miniature excitatory postsynaptic current,mEPSC)频率及幅度的干预作用.方法 取胎鼠(16～18 d)的海马,进行分离纯化和培养,用神经元特异性烯醇化酶(NSE)免疫细胞化学染色法鉴定.随机把海马原代神经元分6组:空白组、模型组、空白血清组、MK-801组、二甲双胍+氟西汀组、左归降糖解郁方组.根据分组,于空白组加10％的培养液;其他5组加15 mmol/L的高糖和50μmol/L的皮质酮予以造模;造模后,空白血清...

Objective To investigate the intervention effects of Zuogui Jiangtang Jieyu Fang on the frequency and amplitude n-methyl-d-aspartic acid receptor(NR)and miniature excitatory postsynaptic current(mEPSC)in hippocampal neurons of fetal rat under simulated diabetes mellitus complicated with depression(DD).Methods The hippocampus of fetal rats(16-18 d)was selected,isolated,purified and cultured.The cultured cells were identified by neuron-specific enolase(NSE)immunocytochemical staining.The hippocampal primary neurons were randomly divided into 6 gr...