期刊论文

Effects of Yiqi Jiedu Formula on Proliferation of Nasopharyngeal Carcinoma CNE2 Cells by Wnt/β-catenin Signaling Pathway

中文

湖南中医药大学学报

1674-070X

2020

40

5

535-539

10.3969/j.issn.1674-070X.2020.05.005

81573721:国家自然科学基金 81874408:国家自然科学基金 81973914:国家自然科学基金 2019JJ40216:湖南省自然科学基金 16C1212:教育厅科学研究项目 2018-072:大学生研究性学习和创新性实验计划

本校为第一机构

目的 探讨益气解毒方对鼻咽癌CNE2细胞增殖及Wnt/β-catenin信号通路的影响.方法 将CNE2细胞用不同浓度(0.25、0.50、1.0 g/L)的益气解毒方水提物和阳性对照药(顺铂,4 mg/L)分别处理24、48、72 h,采用MTT法检测细胞增殖率,流式细胞术检测药物作用48 h后细胞周期分布情况;Western blot检测Wnt/β-catenin信号通路关键蛋白Wnt5a/b、β-catenin及细胞周期蛋白CyclinD1的表达情况.结果 MTT结果显示,益气解毒方对鼻咽癌CNE2细胞增殖具有明显抑制作用,且其抑制作用与作用时间、药物浓度呈正相关(P

Objective To explore the effects of Yiqi Jiedu Formula(YQ)on proliferation of CNE2 cells and Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma.Methods CNE2 cells were respectively treated with different concentrations(0.25,0.50,1.0 g/L)of YQ water extract and positive control medicine(cisplatin,4 mg/L)for 24,48,and 72 h.Then cell proliferation rate activity was detected by MTT method and the cell cycle distribution after 48 h was detected by flow cytometry.Western blot method was used to detect the expression levels of key proteins W...