期刊论文

Effects of Buyang Huanwu Decoction on mTOR Pathway in Cav-1 Knock Out Mice after Cerebral Ischemia

中文

湖南中医药大学学报

1674-070X

2020

40

4

418-422

10.3969/j.issn.1674-070X.2020.04.007

81273989:国家自然科学基金 2018JJ2413:湖南省自然科学基金 2018JJ3383:湖南省自然科学基金 2015140:湖南省中医药局项目

本校为第一机构

目的探讨小窝蛋白（caveolin-1, Cav-1）对脑缺血后哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin, mTOR信号通路的影响及补阳还五汤抗脑缺血的可能作用机制。方法将Cav-1基因敲除（knock out, KO）与野生型（wild type, WT）小鼠随机分为KO假手术组、KO模型组、KO补阳还五汤组（补阳组）、WT假手术组、WT模型组及WT补阳组。采用大脑中动脉栓塞法建立脑缺血模型,干预14 d后观察小鼠神经功能评分;免疫组化检测大脑mTOR、磷酸化核糖体S6蛋白激酶（phospho-ribosomal S6 kinase, p-S6K1）、磷酸化真核细胞翻译起始因子4E结合蛋白-1 （phospho-eukaryotic initiation factor 4E-binding pr...

Objective To investigate the effects of caveolin-1(Cav-1)on mammalian target of rapamycin(mTOR)signaling pathway after cerebral ischemia and the possible mechanism of Buyang Huanwu Decoction(BHD)in anti-cerebral ischemia.Methods Cav-1 knock out mice(KO)and wild type mice(WT)were randomly divided into a KO sham operation group,a KO model group and a KO BHD group,a WT sham operation group,a WT model group,and a WT BHD group.Cerebral ischemia model was established by middle cerebral artery occlusion(MCAO).After 14 days of intervention,the neurological function scores of each group were observed.T...