期刊论文

中文

湖南中医药大学学报

1674-070X

2020

40

2

134-138

10.3969/j.issn.1674-070X.2020.02.003

国家自然科学基金资助项目(81373720)。

本校为第一机构

目的探讨二苯乙烯苷（tetrahydroxy stilbene glycoside, TSG）和远志总皂苷（Tenuigenin, TEN）配伍对Aβ25-35诱导的PC12细胞损伤的影响。方法 PC12细胞除空白组外运用Aβ25-35诱导建立阿尔茨海默病（Alzheimer’s disease, AD）模型,被随机分成模型组、TSG（200 mmol/L）组、TEN组（100 mg/L）、TSG-TEN配伍组（TSG200 mmol/L+TEN100 mg/L）、多奈哌齐组（10μmol/L）。采用MTT比色法检测各组细胞存活率;取细胞上清液分别测各组的乳酸脱氢酶（LDH）、丙二醛（MDA）含量及乙酰胆碱酯酶（AchE）、超氧化物歧化酶（SOD）活力;统计各组的细胞分化率。结果 （1）细胞存活率的高低为:TSG-TEN组>TSG...

Objective To investigate the effects of tetrahydroxy stilbene glycoside(TSG)and tenuigenin(TEN)on the injury of PC12 cells induced by Aβ25-35.Methods PC12 cells were induced by Aβ25-35 to establish a PC12 cell model of Alzheimer’s disease(AD).After modeling,PC12 cells were randomly divided into a blank group,a model group,a TSG(200 mmol/L)group,a TEN group(100 mg/L),a TSG-TEN compatibility group(TSG 200 mmol/L+TEN 100 mg/L)and a donepezil group(10 umol/L).MTT colorimetry was used to detect cell viability in each group;The lactate dehydrogena...