期刊论文

Study on the Intervention Effect and Mechanism of Naotaifang on Neuronal Peroxidation Injury in Rats with Acute Intracerebral Hemorrhage

中文

湖南中医药大学学报

1674-070X

2020

40

7

817-822

10.3969/j.issn.1674-070X.2020.07.008

:国家重点研发计划 2018YFC1704900:中医药现代化研究项目 81774174:国家自然科学基金 81774033:国家自然科学基金 201969:湖南省中医药科研项目

本校为第一机构

目的 研究脑泰方对脑出血急性期大鼠神经细胞过氧化损伤的干预作用及其机制.方法 采用自体血注射法构建SD大鼠脑出血模型,随机分为假手术组、模型组、N-乙酰半胱氨酸(NAC)对照组、脑泰方常规剂量(NTFC)组、脑泰方高剂量(NTFH)组,各组于造模并给药7 d后取材;采用HE染色观察脑组织病理形态,采用Zea Longa 5级评分法进行大鼠神经功能缺失评分,采用生物试剂盒检测病灶脑组织脂质活性氧(lipid-ROS)含量,采用免疫组化及Western blot技术检测谷胱甘肽过氧化物酶-4(GPX-4)及铁死亡标志物环氧合酶-2(COX-2)的表达.结果 与假手术组比较,模型组大鼠脑组织病理损伤明显加重,神经功能缺失评分明显升高,病灶脑...

Objective To study the intervention effect and mechanism of Naotaifang on neuronal peroxidation injury in rats with acute intracerebral hemorrhage.Methods The model of cerebral hemorrhage in SD rats was established by autogenous blood injection, which was divided into a sham operation group, a model group, a N-acetylcysteine(NAC) control group, a Naotaifang conventional dose(NTFC) group and a Naotaifang high dose(NTFH) group.Materials were taken in 7 days after model establishment.HE staining was used to observe the pathological morphology of brain tissue, and Zea longa 5 grade scoring method ...