期刊论文

Discussion on the protective effect of Polygonatum odoratum polysaccharid on mice with D-galactose-induced senescence based on RNA-Seq

中文

湖南中医药大学学报

1674-070X

2022

42

03

367-372

10.3969/j.issn.1674-070X.2022.03.004

湖南省教育厅优秀青年项目（20B441）； 湖南中医药大学校级项目（2021XJJJ016）;湖南中医药大学校级重点项目（2019XJJJ076）；

本校为第一机构

目的 探讨玉竹多糖对D-半乳糖诱导衰老小鼠认知功能保护作用的分子机制。方法 采用水提醇沉法提取玉竹总多糖组分并进行含量测定；将30只昆明小鼠随机分为正常组、模型组、玉竹多糖组（2 g/kg）。模型组及玉竹多糖组小鼠每日皮下注射D-半乳糖（150 mg/kg），正常组及模型组每日灌胃等体积生理盐水，同时玉竹多糖组小鼠灌胃等体积相应剂量的药物。给药8周后处死老鼠并收集海马组织，使用RNA测序（RNA-Seq）分析各组间差异表达的mRNAs，筛选玉竹多糖干预后表达逆转的mRNAs。通过基因本体分析及京都基因与基因组百科全书通路富集分析差异基因参与的主要生物学过程，实时荧光定量PCR（real-time qua...

Objective To explore the molecular mechanism of Polygonatum odoratum polysaccharide on the cognitive function of D-galactose-induced aging mice.Methods The total polysaccharides of Polygonatum odoratum were extracted by water alcohol precipitation method and the content was determined.30 KM mice were randomly divided into normal group,model group,and Polygonatum odoratum polysaccharide group.(2 g/kg).Mice in the model group and Polygonatum odoratum polysaccharide group were injected subcutaneously with D-galactose(150 mg/kg)daily.At the same ti...