期刊论文

中文

湖南中医药大学学报

1674-070X

2023

43

12

2249-2255

10.3969/j.issn.1674-070X.2023.12.015

82174521:国家自然科学基金 2022CX109:湖南中医药大学研究生创新课题立项项目

本校为第一机构

目的 观察推拿㨰法对骨骼肌损伤模型大鼠的机械敏感性离子通道Piezo1 及细胞凋亡的影响,探讨推㨰法治疗骨骼肌损伤的作用机制.方法 32只SD大鼠随机分成正常组、模型组、㨰法组、㨰法+抑制剂组,每组8只.采用肌内注射200 μL虎蛇毒素(notexin,NTX)(10 μg/mL)制备大鼠腓肠肌损伤模型.造模24h后,㨰法组使用实验动物㨰法器模拟㨰法治疗,滚动频率为 140次/min,每天 2 次,每次 3 min,连续治疗 3d;㨰法+抑制剂组在实施㨰法治疗前,给予腹腔注射Piezo1 抑制剂GsMTx4(270 μg/kg),每天 1次,连续注射3d;正常组和模型组腹腔注射等体积生理盐水.采用平衡木测试进行行为学评估,HE染色观察腓肠肌组织显微结构变...

Objective To observe the effects of tuina rolling manipulation on mechanosensitive ion channel Piezo1 and cell apoptosis in skeletal muscle injury model rats,and to explore the mechanism of tuina rolling manipulation in treating skeletal muscle injury.Methods A total of 32 SD rats were randomly assigned into normal group,model group,tuina rolling manipulation group,and tuina rolling manipulation together with inhibitor group,with eight rats in each group.Rat gastrocnemius muscle injury model was prepared by intramuscular injection of 200 μL no...