期刊论文

中文

湖南中医药大学学报

1674-070X

2023

43

12

2177-2184

10.3969/j.issn.1674-070X.2023.12.006

2021108:湖南省中医药科研计划项目 202103051239:湖南省卫健委科研课题 2021JJ30396:湖南省自然科学基金面上项目 2022JJ40335:湖南省自然科学基金青年基金项目

本校为第一机构

目的 观察益肾通癃颗粒对人前列腺癌PC3 细胞荷瘤裸鼠Wnt/β-catenin信号通路的干预作用.方法 利用人前列腺癌骨转移细胞PC3 建立前列腺癌荷瘤裸鼠模型,将其随机分为模型对照组、阳性药物组、益肾通癃颗粒低剂量组、益肾通癃颗粒中剂量组、益肾通癃颗粒高剂量组和联合用药组,给予相应的药物干预,连续 4 周.给药结束后处死动物获取皮下移植瘤瘤体组织样本,分别进行HE染色观察病理变化,免疫组化检测细胞增殖情况,TUNEL检测细胞凋亡情况,Western blot及免疫组织化学法检测Wnt信号通路相关基因蛋白的表达水平.结果 益肾通癃颗粒可以有效改善荷瘤裸鼠皮下移植瘤瘤体组织的病理改变,可以显著抑制肿瘤细...

Objective To observe the intervention effects of Yishen Tonglong Granules(YSTLG)on the Wnt/β-catenin signaling pathway in nude mice bearing human prostate cancer cell PC3 tumor.Methods The models of nude mice bearing prostate cancer tumor were prepared using human prostate cancer bone metastasis cell PC3 and were randomized into model control,positive drug,and low-,medium-,and high-dose YSTLG,and combination drug groups.Corresponding drug interventions were given for four consecutive weeks.After administration,the animals were sacrificed to ob...