期刊论文

中文

湖南中医药大学学报

1674-070X

2024

44

05

737-743

10.3969/j.issn.1674-070X.2024.05.004

82274411:国家自然科学基金 82274412:国家自然科学基金 2022JJ40300:湖南省自然科学基金面上项目 2023JJ30453:湖南省自然科学基金项目 2022XJJB002:湖南中医药大学科研基金揭榜挂帅专项项目 2022RC1021:湖南省青年科技创新人才项目

本校为第一机构

目的 对多柔比星(doxorubicin,DOX)、高血压和异丙肾上腺素(isoproterenol,ISO)诱导的心力衰竭(以下简称"心衰")大鼠模型在造模过程、病理特点等方面进行比较,并对其中医证候进行评价,以助研究者选择适当的模型.方法 30 只雄性Dahl盐敏感性大鼠为高血压心衰模型组(8%NaCl高盐饲料喂养,连续喂养 20 周);60 只雄性SD大鼠随机均分为DOX心衰模型组(DOX 1.5 mg/kg,每周 2 次,腹腔注射,共 7 周)和ISO心衰模型组[ISO 5 mg/(kg·d),连续 7d,腹腔注射,再继续饲养 3 周];另取 10 只雄性SD大鼠作为空白对照组.对大鼠心脏、肾脏、肝脏进行HE染色,对心肌细胞进行Masson染色;用ELISA法检测大鼠血清N末端脑钠肽...

Objective To compare rat models of heart failure induced by doxorubicin(DOX),hypertension,and isoproterenol(ISO)in terms of modelling process and pathological features and to evaluate their Chinese medicine patterns to assist researchers in selecting appropriate models.Methods Thirty male Dahl salt-sensitive rats were used as the hypertensive heart failure model group(8%NaCl high-salt chow fed continuously for 20 weeks);sixty male SD rats were divided randomly and equally into the DOX heart failure model(DOX 1.5 mg/kg,intraperitoneally injected...