期刊论文

中文

湖南中医药大学学报

1674-070X

2024

44

02

235-244

10.3969/j.issn.1674-070X.2024.02.009

20A380:湖南省教育厅重点项目 2022JJ80085:湖南省自然科学基金项目 2022JJ80020:湖南省自然科学基金项目 202113051481:湖南省卫生计生委科研计划课题项目

本校为第一机构

目的 基于GC-MS的血清代谢组学探究夏枯草茎叶酚酸抗慢性盆腔炎的有效成分及其作用机制.方法 本实验采用60只雌性大鼠,采用混合菌液加机械损伤对40只大鼠进行慢性盆腔炎造模处理,正常组10只大鼠不做处理,假手术组10只大鼠只做机械损伤.造模成功后,将模型大鼠分为模型组、康妇炎胶囊组、头孢克洛分散片组和夏枯草茎叶酚酸组,每组10只,连续给药21 d;取正常组、假手术组、模型组和夏枯草茎叶酚酸组、康妇炎胶囊组、头孢克洛分散片组大鼠血清进行代谢组学分析,并对筛选后的差异表达基因进行GO功能分析和KEGG通路富集分析.结果 夏枯草茎叶酚酸能修复慢性盆腔炎组织病理损伤;代谢组学鉴定出47种代谢物...

Objective To explore the active ingredients and mechanism of action of phenolic acids in the stems and leaves of Prunella vulgaris L.against chronic pelvic inflammatory disease based on serum metabolomics using GC-MS.Methods A total of 60 female rats were used in this experiment.Among them,40 rats were treated with mixed bacterial liquid and mechanical injury to establish chronic pelvic inflammatory disease model,ten rats in the normal group were not treated,and ten rats in the sham-operated group were only treated with mechanical injury.After successful modeling,the rat models were subdivided...