期刊论文

中文

湖南中医药大学学报

1674-070X

2024

44

03

357-364

10.3969/j.issn.1674-070X.2024.03.003

kq2202456:长沙市自然科学基金项目 2019XJJJ041:湖南中医药大学校级课题 2022CX143:湖南中医药大学校级课题 2022CX31:湖南中医药大学校级课题

本校为第一机构

目的 观察三补方对自然衰老小鼠认知功能的影响,探讨潜在病理因素和三补方(Sanbu Decoction,SBD)的作用及其可能作用机制.方法 18只 18月龄自然衰老健康SPF级野生型C57BL/6小鼠,雌雄各半,随机分为衰老组(WT-Aged)、多奈哌齐组(WT-Donepezil)、三补方组(WT-SBD),另选 6只 3 月龄小鼠为青年对照组(WT-Young).WT-Young小鼠不灌胃,WT-Aged小鼠用蒸馏水灌胃,其余用相应药物处理,灌胃 45 d.灌胃结束前 6日采用水迷宫实验,记录潜伏期时间、穿越平台次数和平台所在象限游泳路程.用HE染色观察海马神经元形态变化,免疫组织化学法测定小鼠脑海马组织Tau、p-Tau、胶质纤维酸性蛋白(glial fibril-lary acidic...

Objective To observe the effects of Sanbu Decoction(SBD)on the cognitive function of naturally aging mice,and to explore the involved potential pathological factors,as well as the action of SBD and its possible mechanism.Methods Eighteen healthy SPF wild-type C57BL/6 naturally aging mice,half female and half male,aged 18 months,were randomized into aging group(WT-Aged),donepezil group(WT-Donepezil),SBD group(WT-SBD),and another six mice aged three months were selected as the young control group(WT-Young).WT-Young group was not treated.WT-Aged group were treated with distilled water,and the res...