期刊论文

The Mechanism of Composition of Huangqi-danshen on TNFα of Rat Blocked by Nitric Oxide Synthesis

中文

实用预防医学

1006-3110

2002

9

5

478-479

10.3969/j.issn.1006-3110.2002.05.020

国家自然科学基金 (C30 0 0 0 2 2 5)； 国家中医药管理局基础研究项目 (2 0 0 0 - J- T- 1 7)； 湖南省教委青年项目 (0 1 B0 1 4 )资助；

本校为第一机构

目的探讨肿瘤坏死因子α(TNFα)在妊娠高血压综合征及胎儿宫内发育迟缓发病中的作用及益气化瘀法提高胎盘血供的内在机制.方法采用L-精氨酸甲酯(L-NAME)于妊娠第10 d起皮下注射制作模型,黄芪、丹参复方成分及硝酸甘油干预,于妊娠12 d起测尾动脉血压,考马斯亮兰法检测尿蛋白含量,妊娠第18 d用ELISA法检测血浆TNFα水平下降(P＜0.01),而阳性组TNFα水平不仅未降低,反而有所升高,但差异无统计学意义.结论以益气化瘀立法的黄芪丹参复方成分舒张血管、降低外周阻力,改善胎盘循环的作用可能是通过抑制TNFα分泌而实现的.

Objective To explore the role of TNFα played in Pregnancy Induced Hypertension(PIH) and intrauterine growth retardation (IUGR) and the mechanism of the improvement of placenta blood supply by composition of Huangqi-Danshen. Methods On day 10, Hyped rats L-NAME after they were pregnant, and the composistion of Huangqi-Danshen, Glonoin was added by celiac-injection at the meantime. On day 12 of pregnancy, blood pressure of caudal artery and urinary protein level were measured. On day 18 of pregnancy, plasma TNFα content were tested by ELISA. Re...