期刊论文

Nitrogen Oxide Synthesis to Blood Change of Plasma Nitrogen Oxide of Rat Model and Intervention of Yiqi Huayu Principle

中文

实用预防医学

1006-3110

2002

9

6

650-652

10.3969/j.issn.1006-3110.2002.06.030

国家自然科学基金 （C30 0 0 0 2 2 5）； 国家中医药管理局基础研究项目 （2 0 0 0 - J- T- 1 7）； 湖南省教委青年项目 （0 1B0 1 4 ）；

本校为第一机构

目的探讨-氧化氮(NO)在妊娠高血压综合征及胎儿宫内发育迟缓发病中的作用及益气化瘀法提高胎盘血供的内在机制.方法采用L-精氨酸甲酯(L-NAME)于妊娠第10 d起皮下注射制作模型,黄芪、丹参复方成分及硝酸甘油干预,于妊娠12 d起测尾动脉血压,考马斯亮兰法检测尿蛋白含量,妊娠第18 d用亚硝酸根还原酶法检测血浆NO水平;结果模型组NO含量明显降低(P＜0.01),经黄芪丹参复方成分治疗后,血浆NO水平升高(P＜0.01),而阳性组NO含量稍有升高,但差异无统计学意义.结论以益气化瘀立法的黄芪丹参复方成分舒张血管、降低外周阻力,改善胎盘循环的作用可能是通过促进内皮细胞分泌NO而实现的.

Objective To explore the effect of NO in pregnant hypertansion syndrome and fetal intrauterine growth retardation as well as the intrinsic mechanism of YiqiHuoxue principle(YQHYF)in increasing placental blood supply. Methods Applying L-Arginine methylester(L-NAME) on day 10 of model animals' pregnancy, the ingredient of milkvetch and red sage compound recipe and netroglycerine were used to intervene. On day 12 of pregnancy, blood pressure of caudal artery was measured. Urinary protein level was determined, NO level was measured on day 18 with silver nitrite reductase assay. Result The NO level...