期刊论文

中文

实用预防医学

1006-3110

2004

11

4

637-640

10.3969/j.issn.1006-3110.2004.04.001

39770933,30171174:国家自然科学基金 95B063:国家中医药局科研项目 2003034496:中国博士后科学基金 :湖南省高校跨世纪学科带头人培养对象基金

本校为其他机构

目的探讨中药复方益气解毒颗粒抑制鼻咽癌细胞裸鼠移植瘤生长的分子机制. 方法将鼻咽癌细胞系HNE1接种至BABL/c裸小鼠,待移植瘤生长至一定大小(约1 cm×1 cm×1 cm),灌喂益气解毒颗粒药液(按人口服药量换算鼠的给药量),一个疗程(30 d)后处死裸鼠,解剖获取肿瘤组织,测量瘤体体积大小,称量瘤体重量.取100 mg瘤体组织,提取总RNA后逆转录标记成cDNA探针,与588个基因的AtlasTM Human Cancer cDNA Array杂交,灰度扫描杂交信号及统计分析差异. 结果益气解毒颗粒药液能明显抑制鼻咽癌细胞裸鼠移植瘤的生长,灌喂中药组瘤体积和重量分别为(0.207±0.023)cm3和(0.132±0.021)g,对照组为(1.342±0.298)cm3和...

Objective To investigate the inhibitory mechanism of YIQIJIEDU granule on the growth of the implanted tumor of nasopharyngeal carcinoma cell line (HNE1) in nude mice. Methods 5×10 6 HNE1 cells were injected into BABL/c nude mice. Implanted-tumors grew for 20d,whose volume reached 1 cm×1 cm×1 cm. The nude mice were bred YIQIJIEDU granule for 30 d,whose growth was inhibited. The volume and weight of implanted-tumors was measured. The samples were obtained from implanted-tumors,which total RNA was extracted in for gene expression. Reserve trans...