期刊论文

Study on the Effect of the Components of Mongolian Milkvetch Root -Danshen Root Compound on the Expression of Induced Nitrogen Oxide Synthetase (iNOS) mRNA and Monocyte Chemolactic Protein-1 (MCP-1) mRNA on Placental Nutrient Cell of Nitrogen Oxide Synthesis Blocking Pregnant Rats

中文

中国中医药信息杂志

1005-5304

2005

12

5

17-19

10.3969/j.issn.1005-5304.2005.05.008

国家自然科学基金（C30000225）； 国家中医药管理基础研究项目局（2000-J-T-17）； 湖南省教委青年项目（01B014）)；

本校为第一机构

目的研究黄芪丹参复方成分提高胎盘血供的分子机制,为临床有效防治胎盘血供不足所致妊娠并发症提供思路.方法提取分离黄芪、丹参复方成分,运用N0S(一氧化氮合酶)阻滞剂L-精氨酸甲酯(L-NAME)制作一氧化氮合成阻滞大鼠模型,采用原位杂交(ISH)法观察胎盘滋养细胞诱导型一氧化氮合酶(iNOS)、单核细胞趋化蛋白-1(MCP-1)mRNA表达变化及黄芪丹参复方有效成分的影响作用.结果一氧化氮合成阻滞模型组滋养细胞MCP-1 mRNA表达染色明显增强,空白组、黄芪丹参复方成分治疗组及硝酸甘油对照治疗组与模型组比较差异均有显著意义(P＜0.01).模型组iN0S mRNA表达明显增加,与黄芪丹参治疗组、阳性药物硝酸甘油组均有...

the effective components of Mongolian milkvetch root -Danshen root, apply L-Arginine (L-NAME) to pregnant rats to make Nitrogen Oxide Synthesis blocking model. Observe the changes of the expression of iNOS mRNA and MCP-1 mRNA of placental nourish cells and the effection of the components of Mongolian milkvetch root -Danshen root compound on those expressions by the method of in situ hybridization. Results The expression of nourish cells MCP-1 mRNA of model group was obviously dyed deeper. The expression of blank group, treat group, treat group by the components of Mongolian milkvetch root -Dan...