期刊论文

中文

中国中医药信息杂志

1005-5304

2009

16

2

52-53

10.3969/j.issn.1005-5304.2009.02.024

本校为其他机构

目的 建立复方蛇床酊中蛇床子素含量的高效液相色谱测定方法 .方法 采用Hypersil ODS色谱柱(150 mm×4.6 mm,5 μm);流动相为甲醇-水(70∶30),流速为1.0 mL/min;测定波长为322 nm;柱温为室温.结果 蛇床子素在0.018～0.126 μg范围与峰面积呈良好的线性关系(r=0.999 9),平均回收率为98.55%,RSD=1.45%.结论 该方法 操作简便快速、准确可靠,可用于复方蛇床酊中蛇床子素含量的测定.

Objective To establish the determination method for osthol in Compound Shechuang Tincture Methods Hypersil ODS column was used and methanol-water （70 ： 30） was used as a mobile phase. The flow rate was at 1.0 mL/min and the detection wave-length was at 322 nm. Results Osthol had a good linear relationship between 0.018 -0.126μg （r = 0.999 9）. The average recovery was 98.55% and RSD was 1.45%. Conclusion This method is simple, rapid and accurate. It can be applie...