期刊论文

Intervening Effect of Qi-Boosting Toxin-Resolving Formulae in the Developing Process of Nasopharyngeal Carcinogenesis among Rats by Regulating Mitochondrion Pathway

中文

中国中医药信息杂志

1005-5304

2010

17

4

28-30

10.3969/j.issn.1005-5304.2010.04.010

湖南省自然科学基金(07JJ6041) 国家自然科学基金(30572408,30271678)

本校为第一机构

目的 探讨益气解毒方通过调节线粒体途径阻断气虚染毒大鼠鼻咽癌变的发生.方法 72只SD大鼠随机分为模型组、益气解毒方组和维甲酸组.腹腔注射二亚硝基哌嗪(DNP)和佛波醇酯(TPA),同时进行力竭游泳,建立大鼠鼻咽癌变模型,益气解毒方组和维甲酸组分别给予相应药物进行干预治疗,定期分批处死大鼠,取鼻咽组织做病理检测,免疫组织化学SABC法检测蛋白表达,RT-PCR检测mRNA的表达.结果 在诱发性鼻咽癌变进程的单纯性增生阶段,与模型组比较,益气解毒方组和维甲酸组大鼠鼻咽上皮细胞的Bc1-2、Bax、Cytochrome-C和Caspase-3蛋白表达差异均无统计学意义(P>0.05).而在异型增生阶段,益气解毒方组鼻咽上皮细胞的...

Objective To investigate the effects of Qi-Boosting Toxin-Resolving Formulae （QBTRF） by regulating mitochondrion pathway in the developing process of nasopharyngeal carcinogenesis among rats. Methods 72 SD rats were randomly divided into model group, Qi-Boosting Toxin-Resolving Formulae （QBTRF） group, vitamin A acid group. All these animals were subcutaneously injected in axillary fossa with N, N＇dinitrosopiperazine （DNP） and 12-O-tetradecanoylphorbol （TPA） in combination with exhausted swimming to establish the model of nasopharyngeal...