期刊论文

中文

中国中医药信息杂志

1005-5304

2012

19

10

35-37

10.3969/j.issn.1005-5304.2012.10.014

湖南省教育厅重点项目

本校为第一机构

目的观察三物白散药物血清对人胃癌SGC-7901细胞凋亡及survivin mRNA表达的影响。方法选择人胃癌细胞株SGC-7901,分为空白对照组、阳性对照组及三物白散低、中、高剂量组。三物白散药物血清干预SGC-7901细胞后,采用荧光染色法检测胃癌细胞的凋亡率,RT-PCR检测survivin mRNA的表达。结果不同剂量组的三物白散药物血清干预SGC-7901细胞后,与空白对照组比较,各组细胞的凋亡率升高,且细胞凋亡率随药物血清浓度的增大、干预时间的延长逐渐升高;三物白散各剂量组survivin mRNA的表达均有不同程度的下降,与空白对照组比较,差异均有统计学意义(P

Objective To investigate the effect of Sanwubaisan-containing serum on the apoptosis and survivin expression of human gastric cancer cell line SGC-7901.Methods Human gastric cancer cell line SGC-7901 cells were divided into normal group,low-dose Sanwubaisan group,medium-dose Sanwubaisan group,high-dose Sanwubaisan group,positive control group.The apoptosis was measured by fluorescence staining after treated by Sanwubaisan-containing serum,survivin mRNA expression was detected by RT-PCR.Results After the SGC-7901 cells treated by different conce...