期刊论文

中文

中国中医药信息杂志

1005-5304

2013

20

10

25-28

10.3969/j.issn.1005-5304.2013.10.010

国家自然科学基金青年基金（30901921）；

本校为第一机构

目的通过观察咳喘宁对呼吸道合胞病毒(RSV)诱发哮喘模型大鼠CD4~+、CD8~+T细胞内信号转导子和转录激活子6(STAT6)表达的影响,阐明咳喘宁防治RSV诱发支气管哮喘的机理。方法分别从磷酸盐缓冲液(PBS)对照组、模型组大鼠外周血中提取单个核细胞,采用免疫磁珠法分离获得CD4~+、CD8~+T细胞并对其行纯度鉴定与活力检测。采用免疫荧光法,使用流式细胞仪测定T细胞内STAT6蛋白的表达水平,以中药咳喘宁进行干预,动态观察细胞培养过程中,咳喘宁对CD4~+、CD8~+T细胞中STAT6蛋白表达的调节作用。结果免疫磁珠法获得的CD4~+、CD8~+T细胞纯度与活力均支持实验的下行进展。与PBS对照组比较,模型组CD4~+T细胞含量...

Objective To observe the impacts of Kechuanning on expression of signal transducer and activator of transcription 6 （STAT 6） in CD4^＋, CD8^＋T lymphocytes of athma rats induced by virus, and analyze the mechanism of Kechuanning in treating asthma induced by RSV. Methods The PBMC was isolated from the peripheral blood, CD4^＋T, CD8^＋T lymphocyte was separated by immunomagnetic beads. The purity and activity of CD4＋T and CD8＋T lymphocytes were measured by using flow cytometry, trypan-blue dye exclusion test. The protein expression of STAT6 ...