期刊论文

Effects of Simplified Recipe ofBuyang Huanwu Decoction on Expression of APJ in Brain of Focal Cerebral Ischemia Rats

中文

中国中医药信息杂志

1005-5304

2015

9

48-51

10.3969/j.issn.1005-5304.2015.09.014

012CB723503:国家重点基础研究发展计划（973计划） 09k056:湖南省高等学校创新平台开发基金 2013FJ4211:湖南省科技厅项目

本校为第一机构

目的：观察补阳还五精简方对局灶性脑缺血大鼠脑组织APJ蛋白和基因表达的影响，并探讨其作用机制。方法采用大脑中动脉线栓法建立大鼠局灶性脑缺血模型，实验大鼠随机分为假手术组、模型组、补阳还五汤组和补阳还五精简方组，各给药组给予相应药物灌胃。分别于给药第3、7日Western blot、RT-PCR检测各组大鼠脑组织APJ蛋白和基因表达。结果补阳还五精简方组和补阳还五汤组各时间点APJ蛋白和基因表达均高于模型组和假手术组(P＜0.05)，补阳还五精简方组与补阳还五汤组各时间点APJ蛋白表达无明显差异，但补阳还五精简方组APJ基因表达高于补阳还五汤组(P＜0.05)。结论补阳还五精简方可通过促进缺血后...

Objective To observe the effects of simplified recipe ofBuyang Huanwu Decoction on expression of APJ in the brain of local cerebral ischemia rats;To discuss its mechanism of action. MethodsFocal cerebral ischemia rat models were established by middle cerebral arterial occlusion. The adult rats were randomly divided into sham-operation group, model group,Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group. Administration groups were given relevant medicine for gavage. The expressions of APJ protein and APJ mRNA at different time points were detected by Western bl...