期刊论文

Effects of Danshen Tongluo Detoxication Decoction on Inflammatory Response of Rats with Bone Marrow Stem Cell Transplantation in Acute Myocardial Infarction

中文

中国中医药信息杂志

1005-5304

2016

23

11

57-61

10.3969/j.issn.1005-5304.2016.11.014

湖南省教育厅科学研究重点项目（15A143） 湖南省中医药科研计划项目（201559） 国家中医药管理局重点学科开放基金（2014GJXS02）

本校为第一机构

目的观察丹参通络解毒汤对骨髓间充质干细胞(BMSCs)移植急性心肌梗死(AMI)模型大鼠炎症反应的影响,探讨其作用机制。方法采用全骨髓贴壁法结合密度梯度离心法分离、培养BMSCs。结扎冠状动脉左前降支建立AMI模型。大鼠随机分为假手术组、模型组、BMSCs组、丹参通络解毒汤组、丹参通络解毒汤+BMSCs组,每组10只。BMSCs细胞悬液直接注入梗死区边缘心肌组织;各组大鼠给予相应药物或生理盐水灌胃。4周后,ELISA测定大鼠血清单核细胞趋化蛋白-1(MCP-1)、可溶性细胞间黏附因子-1(sICAM-1)含量,Western blot测定心肌组织Toll样受体4(TLR-4)蛋白表达,HE染色观察心肌组织病理形态。结果与模型组比较,BMSCs组...

Objective To explore the effects of Danshen Tongluo Detoxication Decoction on the inflammatory response of rats with bone marrow stem cell (BMSC) transplantation in acute myocardial infarction (AMI); To discuss its mechanism of action. Methods The whole bone marrow adherent method was adopted for BMSCs separation and culture. The AMI model was established by closing the left anterior descending coronary artery of SD rats. After the modeling, SD rats were randomly divided into sham-operation group, model group and BMSCs group (BMSCs transplantation group), Danshen Tongluo Detoxication Decoction...