期刊论文

Comparative Study on Glycosides Content in Liuwei Dihuang Decoction and Cornus Officinalis - Cortex Moutan Couple

中文

中国中医药信息杂志

1005-5304

2018

25

11

57-61

10.3969/j.issn.1005-5304.2018.11.013

201802:湖南省中医药科研项目 2017SK50303:湖南省技术创新引导计划临床医疗技术创新引导项目 ZYYDX201806:湖南中医药大学校级科研基金项目

本校为第一机构

目的 建立HPLC同时测定六味地黄汤及方中山茱萸-牡丹皮药对莫诺苷、獐芽菜苷、芍药苷、马钱苷含量的方法,探讨全方与该药对主要药效成分之间的关联性.方法 采用Hypersil C18色谱柱(4.6 mm× 250 mm,5 μm),流动相为甲醇-水(24∶76),流速1.0 mL/min,检测波长236 nm,柱温30 ℃.结果 莫诺苷、獐牙菜苷、芍药苷和马钱苷分别在0.480～7.680 μg(r＝0.999 3)、0.103～1.650 μg(r＝0.999 5)、0.120～1.920 μg(r＝0.999 1)、0.227～3.630 μg(r＝0.999 7)范围内呈良好的线性关系,平均回收率分别为102.79%、102.29%、100.99%、102.48%,RSD分别为1.73%、1.48%、1.32%、0.75%.六味地黄汤中莫诺苷、獐芽菜...

Objective To establish an HPLC method for the content determination of morroniside, sweroside, paeoniflorin and loganin of Liuwei Dihuang Decoction and its Cornus Officinalis - Cortex Moutan couple; To discuss the relationship between the whole prescription and the couple of main pharmacodynamic components. Methods The HPLC method was used at Hypersile C18 column (4.6 mm × 250 mm, 5 μm); the mobile phase consisted of methanol-water (24:76); the detective wavelength was 236 nm; the flow rate was 1.0 mL/min; the column temperature was 30 ℃. Re...