期刊论文

Study on Protective Mechanism of Panax Notoginseng Saponins on Rats with Renal Ischemia Reperfusion Injury Based on Klotho

中文

中国中医药信息杂志

1005-5304

2018

25

11

31-35

10.3969/j.issn.1005-5304.2018.11.008

国家自然科学基金(81503442) 湖南中医药大学大学生研究性学习和创新性实验计划课题(2017-39) 湖南省教育厅科研项目(17B197)。

本校为第一机构

目的 观察三七总皂苷对肾缺血-再灌注损伤大鼠Klotho蛋白表达的影响,探讨其对模型大鼠的保护机制.方法 实验大鼠随机分为假手术组、模型组、阳性药组和三七总皂苷高、中、低剂量组,各给药组给予相应药物,每日1次.建立大鼠肾缺血-再灌注损伤模型,造模4 h后腹主动脉取血处死大鼠.检测大鼠血清尿素氮(BUN)、肌酐(SCr)水平及肾组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性,HE染色观察肾组织形态变化,免疫组化检测Klotho、核因子-κB(NF-κB) p65蛋白的表达.结果 与假手术组比较,模型组大鼠血清 BUN、SCr 水平明显升高(P＜0.05),肾组织 Klotho蛋白表达降低,NF-κB...

Objective To investigate the effects of Panax Notoginseng saponins (PNS) on protein expression of Klotho in rats with renal ischemia reperfusion injury; To discuss its protective mechanism for model rats. Methods Experimental rats were randomly divided into sham-operation group, model group, positive medicine group, PNS high-, medium- and low-dosage groups. Each administration group was given relevant medicine for gavage, once a day. Renal ischemia reperfusion injury model was established. Rats were sacrificed by taking blood from abdominal aor...